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Fluorescence localization of epidermal cathepsins L and B in the Japanese eel

✍ Scribed by F. Aranishi; N. Mano; H. Hirose


Book ID
110254572
Publisher
Springer
Year
1998
Tongue
English
Weight
56 KB
Volume
19
Category
Article
ISSN
0920-1742

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Cathepsin B was demonstrated cytochemically in human fibroblasts with Z-Ala-Arg-Arg-2-(4-methoxy)naphtylamide as substrate. The enzyme was visualized in the bright field microscope with the diazonium salt Fast Blue B as coupling reagent and in the fluorescence microscope with 5-nitrosalicylaldehyde.