## Abstract We present a time‐correlated single photon counting (TCPSC) technique that allows time‐resolved multi‐wavelength imaging in conjunction with a laser scanning microscope and a pulsed excitation source. The technique is based on a four‐dimensional histogramming process that records the ph
✦ LIBER ✦
Fluorescence lifetime measurements under a microscope by the time-correlated single-photon counting technique
✍ Scribed by Takaaki Minami; Masaki Kawahigashi; Yasuhide Sakai; Katsuyuki Shimamoto; Satoshi Hirayama
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 572 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0022-2313
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## Abstract Multidimensional time‐correlated single photon counting (TCSPC) is based on the excitation of the sample by a high‐repetition rate laser and the detection of single photons of the fluorescence signal in several detection channels. Each photon is characterized by its arrival time in the