Fluorescence labelling of organic acidic compounds with 4-bromomethyl-7-methoxycoumarin (Br-Mmc)
✍ Scribed by Wolfgang Dünges; Axel Meyer; Karl-Erhard Müller; Margit Müller; Rainer Pietschmann; Christoph Plachetta; Rüdiger Sehr; Hedwig Tuss
- Book ID
- 112300316
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 726 KB
- Volume
- 288
- Category
- Article
- ISSN
- 1618-2650
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The use of 4-bromomethyl-7-methoxycoumarin as a derivatizing reagent for fatty acids is examined. The usefulness of the compound for quantitative analysis is limited by the susceptibility of the coumarin ring to base-catalyzed solvolysis. When the derivatization is carried out under conditions which
A sensitive nonradioisotopic method is reported for measuring microsomal lauric acid omega-hydroxylation activity. The assay is based upon separation and detection of 12-hydroxylauric acid formed by means of high-performance liquid chromatography following fluorescence labeling of the carboxyl group