Assay of fatty acid ω-hydroxylation using high-performance liquid chromatography with fluorescence labeling reagent, 3-bromomethyl-7-methoxy-1,4-benzoxazin-2-one (BrMB)

✍ Scribed by Junji Yamada; Mitsuhiro Sakuma; Tetsuya Suga

• Publisher: Elsevier Science
• Year: 1991
• Tongue: English
• Weight: 503 KB
• Volume: 199
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(91)90280-7

✦ Synopsis

A sensitive nonradioisotopic method is reported for measuring microsomal lauric acid omega-hydroxylation activity. The assay is based upon separation and detection of 12-hydroxylauric acid formed by means of high-performance liquid chromatography following fluorescence labeling of the carboxyl group with 3-bromomethyl-7-methoxy-1,4-benzoxazin-2-one (BrMB). The use of 10-hydroxycapric acid as an internal standard affords the accurate and reproducible assay. The differential effect of dehydroepiandrosterone, a peroxisome proliferator, on the omega-hydroxylation activity in the liver and kidney of rats is also reported.