Synthesis and maturation of green fluore
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Vyacheslav A. Kolb; Eugeny V. Makeyev; William W. Ward; Alexander S. Spirin
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Article
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1996
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Springer Netherlands
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English
โ 375 KB
A cell-free translation system producing mature green fluorescent protein (GFP) can be a useful tool for studying the mechanism and kinetics of GFP chromophore formation, as well as for fast protein engineering. We report here that the mature GFP can be formed in the cellfree translation system from