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Fluorescence labeling of the C-terminus of proteins with a puromycin analogue in cell-free translation systems

โœ Scribed by Naoto Nemoto; Etsuko Miyamoto-Sato; Hiroshi Yanagawa


Book ID
117111708
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
262 KB
Volume
462
Category
Article
ISSN
0014-5793

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A cell-free translation system producing mature green fluorescent protein (GFP) can be a useful tool for studying the mechanism and kinetics of GFP chromophore formation, as well as for fast protein engineering. We report here that the mature GFP can be formed in the cellfree translation system from