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Fluorescence-labeled peptides as isoelectric point (pI) markers in capillary isoelectric focusing with fluorescence detection

โœ Scribed by Dr. Kiyohito Shimura; Ken-Ichi Kasai


Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
660 KB
Volume
16
Category
Article
ISSN
0173-0835

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## Abstract A comprehensive twoโ€dimensional (2โ€D) separation system, coupling capillary reverseโ€phase liquid chromatography (cRPLC) to capillary isoelectric focusing (CIEF), is described for protein and peptide mapping. cRPLC, the first dimension, provided highโ€resolution separations for saltโ€free