𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Fluorescence in situ hybridization analysis of whole-arm 7;12 translocations in hematologic malignancies

✍ Scribed by Dr. Bertil Johansson; Kristina Arheden; Mattias Höglund; Annika Othzén; Albert N. Békássy; Ingemar Turesson; Sverre Heim; Felix Mitelman

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
497 KB
Volume
14
Category
Article
ISSN
1045-2257

No coin nor oath required. For personal study only.

✦ Synopsis

Cytogenetic analysis of one case of acute myeloid leukemia (AML), one of acute lymphoblastic leukemia (ALL), one of refractory anemia with excess of blasts (RAEB), and one of acute mixed lineage leukemia (AMLL) with unbalanced 7;12 translocations mapped the breakpoints to the centromeres on both chromosomes. The rearrangements were interpreted as the whole-arm translocations der(7; I2)(q I Oq 10) in the AML and ALL and der(7; I2)(p I0;q 10) in the RAEB and AMLL. However, further analysis by metaphase and/or interphase fluorescence in situ hybridization (FISH) showed centric fusion only in the AML and ALL In the RAEB and AMLL, centromeric material from chromosome 7 but not from 12 was present in the derivative chromosome. Whereas the t(7; 12) resulted in loss of I2p in all four cases, the corresponding chromosome 7 imbalances differecLmonosomy for 7q in the RAEB and AMLL and monosomy for 7p in the AML and ALL. Six hematologic neoplasms with unbalanced whole-arm or near-centromeric 7; I 2 translocations and seven dic(7; I 2) with juxtacentromeric breakpoints have been reported previously: 2 AML, I RAEB in transformation, and 10 ALL. All karyotypically informative cases had loss of I2p material. All but one of the cases with combined 7p and I2p deletion were ALL, whereas all cases with 7q and I2p loss showed myeloid differentiation. No particular clinical, morphologic, or immunophenotypic features seem t o characterize ALLs with t(7; 12). AMLs with an unbalanced t(7; I2), often together with 5q deletions, might be associated with previous genotoxic exposure and poor prognosis. Genes Chromosom Cancer 1456-62 (1995).

📜 SIMILAR VOLUMES

Analysis of deletions of the long arm of
Analysis of deletions of the long arm of chromosome 11 in hematologic malignancies with fluorescence in situ hybridization
✍ Hirofumi Kobayashi; Rafael Espinosa III; Anthony A. Fernald; Catherine Begy; Man 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 540 KB

W e studied samples containing deletions of the long arm of chromosome I I (I I q) from patients with hematologic malignancies by using cytogenetic and fluorescence in situ hybridization (FISH) techniques. Cytogenetic analysis of 28 patients and of a cell line showed that all deletions included band

Identification of new translocations inv
Identification of new translocations involving ETV6 in hematologic malignancies by fluorescence in situ hybridization and spectral karyotyping
✍ María D. Odero; Katrin Carlson; María J. Calasanz; Idoya Lahortiga; Vandana Chin 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 741 KB

## Abstract __TEL__/__ETV6__ is the first transcription factor identified that is specifically required for hematopoiesis within the bone marrow. This gene has been found to have multiple fusion partners; 35 different chromosome bands have been involved in __ETV6__ translocations, of which 13 have

Simultaneous detection of MYC, BVR1, and
Simultaneous detection of MYC, BVR1, and PVT1 translocations in lymphoid malignancies by fluorescence in situ hybridization
✍ Katrina Rack; Eric Delabesse; Isabelle Radford-Weiss; Priscille Bourquelot; Gaël 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 232 KB 👁 2 views

The rapid detection of chromosome band 8q24 rearrangements, including classical translocations involving MYC and variant 3Ј translocations, is important for the accurate diagnosis and appropriate treatment of lymphoid malignancies. We have identified and characterized a CEPH YAC, 934e1, which extend

Chromosomal in situ suppression hybridiz
Chromosomal in situ suppression hybridization of immunologically classified mitotic cells in hematologic malignancies
✍ Marianne Tiainen; Sakari Knuutila; Susanne Popp; Patricia Emmerich; Thomas Creme 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 646 KB

Chromosomal in situ suppression (CISS) hybridization was performed with library DNA from sorted human chromosomes 8, 9, IS, 17, 2 I, and 22 on immunologically stained bone marrow cells of four patients with a hematologic neoplasm, including two patients with myelodysplastic syndrome and trisomy 8, o