𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Fluorescence detection techniques for protein kinase assay

✍ Scribed by YongJin Li; WeiHong Xie; GuiJie Fang

Publisher
Springer
Year
2008
Tongue
English
Weight
313 KB
Volume
390
Category
Article
ISSN
1618-2650

No coin nor oath required. For personal study only.

πŸ“œ SIMILAR VOLUMES

A continuous fluorescence assay for prot
A continuous fluorescence assay for protein kinase C
✍ Brian K. McIlroy; John D. Walters; J.David Johnson πŸ“‚ Article πŸ“… 1991 πŸ› Elsevier Science 🌐 English βš– 553 KB

A 6-acryloyl-2-dimethylaminonapthalene (acrylodan)-labeled 25-amino acid peptide (acrylodan-CKK-KKRFSFKKSFKLSGFSFKKNKK-COO-), containing the protein kinase C (PKC) phosphorylation sites of brain myristoylated alanine-rich kinase C substrate protein, undergoes a 20% fluorescence decrease when it is p

Mechanism-Based Fluorescent Reporter for
Mechanism-Based Fluorescent Reporter for Protein Kinase A Detection
✍ Benedict Law; Ralph Weissleder; Ching-Hsuan Tung πŸ“‚ Article πŸ“… 2005 πŸ› John Wiley and Sons 🌐 English βš– 213 KB

## Abstract __A novel mechanism‐based fluorescent reporter was designed for the detection of protein kinase A (PKA), which is known to mediate a variety of cellular responses in most eukaryotic cells. The probe consists of a specific binding peptide sequence, LRRRRFAFC, conjugated with 2′‐thioethyl

Feasibility studies on a protein kinase
Feasibility studies on a protein kinase assay when using radioisotope detection technique for developing a protein biochip
✍ Sang Hyun Park; Kyong-Cheol Ko; Hui Jeong Gwon πŸ“‚ Article πŸ“… 2007 πŸ› John Wiley and Sons 🌐 French βš– 192 KB

## Abstract Microarrays have recently become a precious research tool for proteomics and clinical investigation. Their applications to the diagnosis of a disease have emerged as a significant promise for medical advances. In this study, we report on an efficient strategy for the detection of phosph

A microtiter-based assay for the detecti
A microtiter-based assay for the detection of protein tyrosine kinase activity
✍ Jeffrey S. Cleaveland; Peter A. Kiener; David J. Hammond; Bernice Z. Schacter πŸ“‚ Article πŸ“… 1990 πŸ› Elsevier Science 🌐 English βš– 505 KB

A 96-well microtiter enzyme-linked immunosorbent assay (ELISA) for protein tyrosine kinases has been developed. This assay uses one of several substrates that are phosphorylated by tyrosine kinase, an antibody to phosphotyrosine, and a peroxidase-linked second antibody. Color development is monitore