Feasibility studies on a protein kinase assay when using radioisotope detection technique for developing a protein biochip

✍ Scribed by Sang Hyun Park; Kyong-Cheol Ko; Hui Jeong Gwon

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: French
• Weight: 192 KB
• Volume: 50
• Category: Article
• ISSN: 0022-2135
• DOI: 10.1002/jlcr.1416

✦ Synopsis

Abstract

Microarrays have recently become a precious research tool for proteomics and clinical investigation. Their applications to the diagnosis of a disease have emerged as a significant promise for medical advances. In this study, we report on an efficient strategy for the detection of phosphorylation of a substrate catalyzed by kinase, using the radioisotope (RI) detection technique for a protein biochip. This technique does not employ the use of the blocking step which is commonly used in conventional methods to prevent non‐specific binding. It was found that the usage of a RI detection technique has the advantages of being highly sensitive and time saving when compared with other conventional methods. The results can be applied when using RI detection technique to develop biochips to determine the activity of a protein kinase. Further, it can be a useful tool for a high throughput screening and for studying protein–protein interactions. Copyright © 2007 John Wiley & Sons, Ltd.