✦ LIBER ✦

Fluorescence detection of enzymatically formed hydrogen peroxide in aqueous solution and in reversed micelles

✍ Scribed by Alvaro Sanchez Ferrer; Jillert S. Santema; Riet Hilhorst; Antonie J.W.G. Visser

Publisher: Elsevier Science
Year: 1990
Tongue: English
Weight: 419 KB
Volume: 187
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(90)90429-d

No coin nor oath required. For personal study only.

✦ Synopsis

A sensitive enzymatic assay for oxidase reactions both in aqueous solution and in hexadecyltrimethylammoniumbromide (CTAB) reversed micelles has been developed. The assay is based on the fluorescence detection of dichlorofluorescein, which is formed by hydrogen peroxide oxidation of the nonfluorescent precursor dichlorofluorescin. Hydrogen peroxide as product of the reaction catalyzed by glucose oxidase served to select the reaction conditions. The reaction rate is distinctly enhanced in CTAB reversed micelles as compared to the rate in aqueous solution. This effect, combined with the high sensitivity owing to the strong fluorescence of dichlorofluorescein, makes the assay attractive for the detection of low enzyme, substrate, or peroxide concentrations.

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