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Fluorescence derivatization approaches for quantitative high-performance liquid chromatography of peptides

โœ Scribed by Gerald R. Rhodes; Venkata K. Boppana; Marc J. Rubenfield

Book ID
119133827
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
519 KB
Volume
13
Category
Article
ISSN
0168-3659

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A highly sensitive peptide mapping method using derivatization and fluorescence detection is described. Bovine cytochrome c was digested using a buffer compatible with the derivatization that followed. The derivatization was performed with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate. The peptide