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Fluorescence correlation spectroscopy as a tool for measuring the rotational diffusion of macromolecules

✍ Scribed by Christoph M. Pieper; Jörg Enderlein

Book ID
113554957
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
922 KB
Volume
516
Category
Article
ISSN
0009-2614

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Fluorescence correlation spectroscopy in the nanosecond time range: rotational diffusion of bovine carbonic anhydrase B
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A fluorescence correlation experiment for measurement of rotational diffusion in the nanosecond time scale is described. Using this method, the rotational diffusion coefficient of bovine carbonic anhydrase B labelled with tetramethylrhodamine isothiocyanate was estimated to be Dr = (1.14 +/- 0.15) X