Fluorescence correlation spectroscopy in the nanosecond time range: rotational diffusion of bovine carbonic anhydrase B
✍ Scribed by P. Kask; P. Piksarv; Ü. Mets; M. Pooga; E. Lippmaa
- Publisher
- Springer
- Year
- 1987
- Tongue
- English
- Weight
- 426 KB
- Volume
- 14
- Category
- Article
- ISSN
- 1432-1017
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✦ Synopsis
A fluorescence correlation experiment for measurement of rotational diffusion in the nanosecond time scale is described. Using this method, the rotational diffusion coefficient of bovine carbonic anhydrase B labelled with tetramethylrhodamine isothiocyanate was estimated to be Dr = (1.14 +/- 0.15) X 10(7) s-1 at 22 degrees C. The experiment is based on a cw argon ion laser, a microfluorometer with local solution flow inside the sample cell, and two photon detectors. The fluorescence intensity autocorrelation function in the nanosecond time range is computed with the help of a time-to-amplitude converter and a multichannel pulse-amplitude analyser.