Fluorescence Microplate-Based Assay for
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Laurie J. Jones; Victoria L. Singer
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Article
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2001
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Elsevier Science
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English
β 112 KB
We have developed a simple, sensitive, fluorescence microplate-based assay for tumor necrosis factor (TNF) biological activity. The assay employs SYTOX Green nucleic acid stain to detect TNF-induced cell necrosis in actinomycin D sensitized cultured cell lines. SYTOX Green stain is a cationic unsymm