A continuous assay was developed for processive DNA polymerases. The specific enzyme used to develop the assay was the most processive polymerase known, Escherichia coli DNA polymerase III holoenzyme. The assay was based upon the recovery of the intrinsic fluorescence of single-stranded DNA binding
Fluorescence-Based DNA Polymerase Assay
β Scribed by Heidi Tveit; Tom Kristensen
- Publisher
- Elsevier Science
- Year
- 2001
- Tongue
- English
- Weight
- 53 KB
- Volume
- 289
- Category
- Article
- ISSN
- 0003-2697
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