✦ LIBER ✦

A Solid-Phase Assay for Thermophilic DNA Polymerases

✍ Scribed by D.J. Day; D.J. Saul; R.A. Reeves; P.L. Bergquist

Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 256 KB
Volume: 211
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.1993.1251

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Fluorescence Recovery Assay: A Continuou

Fluorescence Recovery Assay: A Continuous Assay for Processive DNA Polymerases Applied Specifically to DNA Polymerase III Holoenzyme

✍ Mark A. Griep 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 153 KB

A continuous assay was developed for processive DNA polymerases. The specific enzyme used to develop the assay was the most processive polymerase known, Escherichia coli DNA polymerase III holoenzyme. The assay was based upon the recovery of the intrinsic fluorescence of single-stranded DNA binding

A rapid and accurate procedure for assay

A rapid and accurate procedure for assaying DNA polymerase, RNA polymerase, or ribosome dependent protein synthesis

✍ Bernard I. Weinstein; Nina Bhardwaj; Heng-Chun Li 📂 Article 📅 1975 🏛 Elsevier Science 🌐 English ⚖ 384 KB

A descending paper chromatographic procedure for separating trichloracetic acid soluble from precipitable material is described. Its use for assaying DNA, RNA, or protein synthesis from a variety of sources with various states of purity attests to its general applicability to reactions of interest t

Description of a Solid Phase Enzymoassay

Description of a Solid Phase Enzymoassay for the Detection of Reverse Transcriptase, DNA Polymerase, and Deoxyribonuclease

✍ M. Nocito; L.M. Villar; P. Gonzalezporque 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 243 KB

Nucleotide analogues as probes for DNA p

Nucleotide analogues as probes for DNA polymerases

✍ K.-H. Jung; A. Marx 📂 Article 📅 2005 🏛 Springer 🌐 English ⚖ 608 KB

The polymerase inhibition assay: A metho

The polymerase inhibition assay: A methodology for the identification of DNA-damaging agents

✍ Gareth J. S. Jenkins; Brian Burlinson; James M. Parry 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 162 KB

We report here the development of the polymerase inhibition assay (PI assay), a methodology capable of simultaneously identifying multiple DNA-damaging agents. The PI assay was developed in order to fulfil a requirement for the screening of new pharmaceuticals for potential DNA-damaging effects. The

Monoclonal antibody-mediated solid-phase

Monoclonal antibody-mediated solid-phase assay for mammalian O6-alkylguanine DNA alkyltransferase activity

✍ Masahiko S. Satoh; Chieko Moriyama; Akira Asai; Hiroshi Handa; Manfred F. Rajews 📂 Article 📅 1991 🏛 Elsevier Science 🌐 English ⚖ 787 KB

We describe a sensitive, rapid, and simple assay for mammalian O6-alkylguanine DNA alkyltransferase (O6-AGT) utilizing solid-phase DNA as the substrate and a monoclonal antibody (Mab)-based immuno-slotblot (ISB) for quantitation of O6-ethylguanine (O6-EtG). lambda-phage DNA was treated with N-ethyl-