Fluorescence-based biosensing of zinc using carbonic anhydrase
β Scribed by Carol A. Fierke; Richard B. Thompson
- Book ID
- 110317988
- Publisher
- Springer Netherlands
- Year
- 2001
- Tongue
- English
- Weight
- 249 KB
- Volume
- 14
- Category
- Article
- ISSN
- 1572-8773
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A new type of fluorescence transduction method for determining zinc in solution is described. The approach is based upon energy transfer from a fluorescent label on an enzyme, human carbonic anhydrase II, to a colored inhibitor which binds to zinc in the enzyme active site. If zinc is present in sol
## Abstract A practical laboratory experiment is described that illustrates the application of fluorescence resonance energy transfer to the study of proteinβligand binding. The affinities of wildβtype and mutant human carbonic anhydrase II for dansylamide were determined by monitoring the increase