Fluorescein conjugated α-Bungarotoxin: Its properties and interaction with acetylcholine receptors

✍ Scribed by J.B. Suszkiw; M. Ichiki

• Book ID: 102629043
• Publisher: Elsevier Science
• Year: 1976
• Tongue: English
• Weight: 353 KB
• Volume: 73
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(76)90146-9

✦ Synopsis

ACh receptor specific a-Bungarotoxin ((u-BGT) can be coupled with FITC to yield two fluorescein (Y-BGT derivatives, one with an isoelectric point of 7.1 (F-(rBGT-~1-7.1) and the other with the isoelectric point of 5.2 (F-(YBGT-pl-5.2). Only F-cuBGT-~1-7.1 is suitable for fluorescent staining of cholinergic receptors. It is approximately six to nine times less potent than the native toxin and is only slowly reversible. The F-o(-BGT-~1-5.2 is about 145 times less potent than the native toxin and is reversible.