A modification of α-bungarotoxin amino group residues with retention of binding properties to isolated and membrane-bound acetylcholine receptor

A simple quantitative assay of 125I-labe

A simple quantitative assay of 125I-labeled α-bungarotoxin binding to soluble and membrane-bound acetylcholine receptor protein

✍ Ronald A. Kohanski; John P. Andrews; Pierre Wins; Mohyee E. Eldefrawi; George P. 📂 Article 📅 1977 🏛 Elsevier Science 🌐 English ⚖ 535 KB

This method involves the irreversible formation of a complex between ?labeled cu-bungarotoxin and the acetylcholine receptor in either its membranebound or purified state. The separation of the labeled toxin-receptor complex from unreacted toxin is accomplished by chromatography on carboxymethylcell