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Flow-through systems for culturing great scallop larvae

✍ Scribed by Sissel Andersen; Gavin Burnell; Øivind Bergh


Book ID
110273396
Publisher
Springer Netherlands
Year
2000
Tongue
English
Weight
78 KB
Volume
8
Category
Article
ISSN
0967-6120

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A flow-through system in which monolayer cells, growing on a 50-cm2 glass surface, are in contact with a film of medium with a thickness of 0.14 mm, is described. For murine B16 melanoma cells, the loading capacity is 8. lo6 cells. The flow-through principle permits frequent off-line or on-line dete