✦ LIBER ✦

A flow-through tissue culture system with fast dynamic response

✍ Scribed by J. Lankelma; E. Laurensse; H.M. Pinedo

Publisher: Elsevier Science
Year: 1982
Tongue: English
Weight: 508 KB
Volume: 127
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(82)90184-1

No coin nor oath required. For personal study only.

✦ Synopsis

A flow-through system in which monolayer cells, growing on a 50-cm2 glass surface, are in contact with a film of medium with a thickness of 0.14 mm, is described. For murine B16 melanoma cells, the loading capacity is 8. lo6 cells. The flow-through principle permits frequent off-line or on-line detection of medium constituents for a period on the order of days. The system has a fast dynamic response. With off-line radiochemical detection, the system was applied to the uptake of uridine and excretion of uracil over a period of 45 h. With on-line fluorescence detection, the interaction between the cells and two anthracycline analogs was monitored. The cells can be easily observed with a light microscope.

📜 SIMILAR VOLUMES

Flow dynamics within a bioreactor for ti

Flow dynamics within a bioreactor for tissue engineering by residence time distribution analysis combined with fluorescence and magnetic resonance imaging to investigate forced permeability and apparent diffusion coefficient in a perfusion cell culture chamber

✍ Justin Dubois; Luc Tremblay; Martin Lepage; Patrick Vermette 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 637 KB

## Abstract This study reveals that residence time distribution (RTD) analysis with pH monitoring after acid bolus injection can be used to globally study the flow dynamics of a perfusion bioreactor, while fluorescence microscopy and magnetic resonance imaging (MRI) were used to locally investigate