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Flow karyotype analysis and fluorescence-activated sorting of burkitt-lymphoma-associated translocation chromosomes

✍ Scribed by Zvi Wirschubsky; Catharina Perlmann; Jan Lindsten; George Klein

Publisher
John Wiley and Sons
Year
1983
Tongue
French
Weight
640 KB
Volume
32
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

Flow karyotype analysis was performed to assess the feasibility of fluorescence‐activated sorting of Burkitt lymphoma (BL)‐associated translocation chromosomes. The typical 14q+ chromosome in the t(8;14) and the two “variant translations”, the 2q+ in the t(2;8) and the small 22q‐ in the t(8;22), could be identified as single peaks within the flow karyotypes of metaphase chromosomes isolated from several different BL‐lines for each translocation. The translocation chromosomes could be separated with a high degree of purity and in quantities suitable for biochemical analysis. The same analytical and preparative technique was also successfully applied to the identification and sorting of the Philadelphia (Ph~1~) chromosome in a 9;22 translocation‐carrying CML‐derived line and a familial (11;22) translocation.

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