Study of X chromosome abnormality in XX males using bivariate flow karyotype analysis and flow sorted dot blots

✍ Scribed by N. P. Carter; M. E. Ferguson-Smith; N. A. Affara; H. Briggs; M. A. Ferguson-Smith

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 586 KB
• Volume: 11
• Category: Article
• ISSN: 0196-4763
• DOI: 10.1002/cyto.990110123

✦ Synopsis

We have used bivariate flow karyotype analysis to quantify aberrant X chromosome size in 11 XX males. With one exception, the patients could be grouped into those with an X homologue difference greater than normal (Group A, n = 3) and into those whose X homologue difference could not be distinguished from female controls (Group B, n = 7). The range of sizes of the aberrant X chromosome in Ysequence positive patients agrees with the variable nature of the X-Y interchange in these individuals as determined by the use of Y-specific DNA probes and Southern blotting analysis. In one patient it was possible to sort separately the normal and the X-Y inter-changed homologues for dot blot analysis. The presence of Y sequences and an increased dose of the zinc finger gene, ZFY, were detected in the X-Y interchanged homologue. In preliminary studies of 5 male and 6 female controls, it was noted that a consistent difference between the two X homologues in females was found which could not be totally explained by errors of the fitting procedure. We suggest that this difference could be due to X inactivation and that the two X homologues in females might be distinguishable.