Flow injection cytometry: A new approach for sample and solution handling in flow cytometry

✍ Scribed by Walter Lindberg; Jaromir Ruzicka; Gary D. Christian

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 711 KB
• Volume: 14
• Category: Article
• ISSN: 0196-4763
• DOI: 10.1002/cyto.990140217

✦ Synopsis

A prototype instrument for sample and solution handling in flow cytometry has been constructed. The system is modular and allows the control of any combination of up to 4 pumps, 2 selection valves, and 2 injection valves. These devices are controlled by a computer using TTLlogic. The flow injection instrument is interfaced to the flow cytometer via a 6- port injection valve, thereby facilitating virtually any flow pattern to be implemented without affecting the fluidics of the cytometer. Salient features of the instrument are accurate control of the volume of injected sample and reagent, reproducible timing, and controlled mixing conditions. Results from model experiments of on-line staining of trout erythrocytes with different concentrations of 4',6-diamidino-2-phenylindole (DAPI) and propidium iodide (PI) are shown. Possible ways to improve the performance and utility of the instrument are also discussed. o 1993 Wiley-Liss, Inc.