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Flow-injection chemiluminescent determination of glycerol-3-phosphate and glycerophosphorylcholine using immobilized enzymes

✍ Scribed by Yaqoob, M. ;Nabi, A. ;Masoom-Yasinzai, M.

Publisher: John Wiley and Sons
Year: 1997
Weight: 76 KB
Volume: 12
Category: Article
ISSN: 0884-3996
DOI: 10.1002/(sici)1099-1271(199701/02)12:1<1::aid-bio425>3.0.co;2-r

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✦ Synopsis

Flow injection procedures with immobilized enzyme mini-columns are described for the determination of glycerol-3-phosphate, and glycerophosphorylcholine with chemiluminescent detection. The hydrogen peroxide produced on-line is coupled with a luminol (5-amino-2,3,-dihydro-1,4-phthalazinedione) peroxidation chemiluminescent system. The detection limits for glycerol-3-phosphate and glycerophosphorylcholine are 5 ؋ 10 Ϫ 7 M and 1 ؋ 10 Ϫ 6 M respectively with r.s.d. < 2%. The sample throughput is 40/h. The immobilized enzyme columns did not show any deterioration in activity after usage for 3 months.

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