Flow injection determination of glucose, bile acid and ATP using immobilized enzyme reactor and chemiluminescent assay of NAD(P)H

✍ Scribed by Maeda, Masako ;Tsuji, Akio ;Ohshima, Nobuo ;Hukuoka, Masayoshi

• Publisher: John Wiley and Sons
• Year: 1993
• Weight: 407 KB
• Volume: 8
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/bio.1170080503

✦ Synopsis

We have developed a chemiluminescent f l o w injection method for analysis o f bile acid, glucose and ATP using the chemiluminescent assay o f NADH using 1 -methoxy-5methylphenazinium methyl sulphate (1 -MPMS)/isoluminol(lL)/microperoxidase (m-POD) system and immobilized enzyme reactors such as 3a-hydroxysteroid dehydrogenase, glucosedehydrogenase, hexokinase and glucose-6-phosphate dehydrogenase. The standard curves were obtained in the range o f 5 -l 0 0 p m o l for bile acid, 0.5 N 5.0nmol for glucose and lo-'mol/L for ATP. The coefficient o f variation far each assay was not more than 4.1% for bile acid, 2.3% for glucose and 5.3% for ATP, respectively.