A high-performance liquid chromatographic method with fluorescence detection was developed for a series of cholinergic channel activators using 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-F) as a fluorescent labeling reagent. This method includes three separate steps: extraction of parent compound
Flow Injection Analysis of Some Nonapeptides Derivatized with 4-Fluoro-7-Nitrobenzo-2,1,3-Oxadiazole
โ Scribed by W. R. G. Baeyens; G. Van der Weken; Y. Vanden Bossche; A. van Overbeke; K. Imai
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 107 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0269-3879
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The simultaneous determination of amino acid composition including cysteine of egg albumin, a model protein containing a/s cysteine residue, is reported. All the thiol groups of the cysteine residue@) of egg albumin were labelled with 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole, a fluorogenic re