We investigated cyclin B1 expression during the cell cycle in human glioma cells cultured under asynchronous growing condition by two cycometry techniques: flow cytometry (FCM) and laser scan-
Flow Cytometry Study of Human Cyclin B1 and Cyclin E Expression in Leukemic Cell Lines: Cell Cycle Kinetics and Cell Localization
β Scribed by Jean-Francois Viallard; Francis Lacombe; Maryse Dupouy; Helene Ferry; Francis Belloc; Josy Reiffers
- Book ID
- 115603174
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 231 KB
- Volume
- 247
- Category
- Article
- ISSN
- 0014-4827
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## Flow cytometric cyclin expression/DNA content analysis, now commonly used, provides useful information on the mechanisms regulating cell cycle progression. However, this biparametric analysis does not make a clear-cut distinction between G 1 and S-early or between S-late and G 2 M phase cells.
Cyclins are important in the regulatory system controlling cell cycle progression and overexpression or aberrant expression of G1 cyclins, as cyclin E, may result in an inadequate G1-S transition. In this study, the expression of cyclin E was analyzed in 12 human cell lines and 18 hematological mali