Flow cytometry measurements of human chromosome kinetochore labeling
β Scribed by Judith A. Fantes; D. K. Green; Patricia Malloy; A. T. Sumner
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 775 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0196-4763
No coin nor oath required. For personal study only.
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## Abstract A method of preparation and flow cytometric analysis of chromosomes from human peripheral lymphocytes is described. The procedure allows a resolution coefficient of variation better than 3% using propidium iodide staining and a commercially available flow cytometer.
Certain variables in the preparation of chromosome suspensions for flow cytometric analysis have been investigated. The optimal conditions have been determined. The results of this series of experiments have been incorporated to yield a preparation protocol that gives chromosome profiles with a low