Background: A flow cytometric measurement (FCM) technique has been developed to size DNA fragments. Individual fragments of a restriction digest of genomic DNA, stained with an intercalating dye, are passed through an ultrasensitive cytometer. The measured fluorescence intensity from each fragment i
β¦ LIBER β¦
Flow cytometry and bacterial pathogenesis
β Scribed by Raphael H Valdivia; Stanley Falkow
- Book ID
- 117765202
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 510 KB
- Volume
- 1
- Category
- Article
- ISSN
- 1369-5274
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The ability of a flow cytometer to rapidly assess microbial viability was investigated using three vital stains: rhodamine 123 (Rh123); 3,3'-dihexyloxacarbocyanine iodide [DiOC6(3)] and fluorescein diacetate (FDA). Rh123 was found to clearly differentiate viable from non-viable bacteria. The methodo