Quantification of bacterial phagocytosis by flow cytometry and spectrofluorimetry
โ Scribed by Nathalie Plasman; Bernard Vray
- Book ID
- 118902912
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 676 KB
- Volume
- 174
- Category
- Article
- ISSN
- 0022-1759
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Background: A flow cytometric measurement (FCM) technique has been developed to size DNA fragments. Individual fragments of a restriction digest of genomic DNA, stained with an intercalating dye, are passed through an ultrasensitive cytometer. The measured fluorescence intensity from each fragment i
The ability of a flow cytometer to rapidly assess microbial viability was investigated using three vital stains: rhodamine 123 (Rh123); 3,3'-dihexyloxacarbocyanine iodide [DiOC6(3)] and fluorescein diacetate (FDA). Rh123 was found to clearly differentiate viable from non-viable bacteria. The methodo