✦ LIBER ✦

Flow cytometric study of changes in the intracellular free calcium during the cell cycle

✍ Scribed by Gopal Pande; N. Anil Kumar; P. S. Manogaran

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 770 KB
Volume: 24
Category: Article
ISSN: 0196-4763
DOI: 10.1002/(sici)1097-0320(19960501)24:1<55::aid-cyto7>3.0.co;2-h

No coin nor oath required. For personal study only.

✦ Synopsis

We measured the intracellular levels of free cytoplasmic calcium in Werent phases of the cell cycle in viable rat fibroblasts, using two parameter flow cytometric analysis with Hoechst 33342 as the DNA specific dye and Fluo-3 as the calcium sensitive dye. We studied changes in calcium levels during the G1 phase of cell cycle by arresting cells with chemical agents such as stamsporine and hydmxyurea or by density dependent arrest of cell growth. We show that levels of calcium are lowest at the beginning of G1 phase but rise steadily with its progression and culminate at the Gl/S border. Our results suggest that complex changes occur in calcium levels dur-ing the process of mitotic division. During progression of S and G2 phases, calcium levels decline and increase respectively. Our results offer a new methodology to estimate intracellular calcium levels in specific phases of the cell cycle. Based upon these results we propose a general scheme representing the changes in the intracellular calcium concentration during the progression of the cell cycle.

📜 SIMILAR VOLUMES

Flow cytometric analysis of the cell cyc

Flow cytometric analysis of the cell cycle in polyamine-depleted cells

✍ Pirkko Pohjanpelto; Stig Nordling; Sakari Knuutila 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 707 KB

Flow cytometric study of the type II pne

Flow cytometric study of the type II pneumocyte cell cycle in vivo and in vitro

✍ Bruce D. Uhal 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 737 KB

Changes in intracellular calcium concent

Changes in intracellular calcium concentration and pH of target cells during the cytotoxic process: A quantitative study at the single cell level

✍ Katarina Radoškević; Bart G. de Grooth; Jan Greve 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 844 KB

Changes in the rate of RNA synthesis dur

Changes in the rate of RNA synthesis during the cell cycle

✍ Salem, Cherine ;El-Alfy, Mohamed ;Leblond, Charles Philippe 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 126 KB 👁 2 views

## Background: Although the rate of rna synthesis is known to drop at mitosis, the recent identification of 11 stages in the cell cycle (el-alfy et al., 1994) makes it possible to measure the rate of this synthesis at each one of the stages and thus find out how it varies throughout the cell cycle.

Intracellular localization of cyclin B1

Intracellular localization of cyclin B1 during the cell cycle in glioma cells

✍ Shunsuke Kakino; Kohsuke Sasaki; Akira Kurose; Haruhide Ito 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 779 KB

We investigated cyclin B1 expression during the cell cycle in human glioma cells cultured under asynchronous growing condition by two cycometry techniques: flow cytometry (FCM) and laser scan-

Flow cytometric analysis of protein thio

Flow cytometric analysis of protein thiol groups in relation to the cell cycle and the intracellular content of glutathione in rat hepatocytes

✍ P. Principe; G. D. Wilson; P. A. Riley; T. F. Slater 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 944 KB