Flow cytometric reticulocyte analysis using thiazole orange; clinical experience and technical limitations

Heterogeneity of peripheral blood reticu

Heterogeneity of peripheral blood reticulocytes: A flow cytometric analysis with monoclonal antibody HAE9 and thiazole orange

✍ Eugene B. Mechetner; Daniel D. Sedmak; Rolf F. Barth 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 222 KB

## Abstract The expression of a human erythroid cell surface antigen recognized by monoclonal antibody (mAB) HAE9 has been studied on peripheral blood reticulocytes by one‐ and two‐color flow cytometry. Total reticulocyte count was determined using Thiazole Orange (TO) and flow cytometry. In normal