𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Heterogeneity of peripheral blood reticulocytes: A flow cytometric analysis with monoclonal antibody HAE9 and thiazole orange

✍ Scribed by Eugene B. Mechetner; Daniel D. Sedmak; Rolf F. Barth

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
222 KB
Volume
38
Category
Article
ISSN
0361-8609

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The expression of a human erythroid cell surface antigen recognized by monoclonal antibody (mAB) HAE9 has been studied on peripheral blood reticulocytes by one‐ and two‐color flow cytometry. Total reticulocyte count was determined using Thiazole Orange (TO) and flow cytometry. In normal individuals, 4.56% of reticulocytes were stained by FITC‐labeled mAB HAE9. The correlation between reticulocyte percentage by TO and HAE9 staining was 0.828 (P < 0.0001) in patients with hematocrits less than 0.25. A HAE9‐positive reticulocyte percentage of 6–44% was observed when analyzed by two‐color flow cytometry with TO and mAB HAE9. These findings, in conjunction with previous studies, suggest that mAB HAE9 recognizes an early, less differentiated population of peripheral blood reticulocytes. Enumeration of immature reticulocytes may be of clinical utility.

📜 SIMILAR VOLUMES

Flow cytometric analysis of the ploidy o
Flow cytometric analysis of the ploidy of normoblasts in the peripheral blood of patients with beta-thalassemia
✍ Dr. Eitan Fibach; Eliezer A. Rachmilewitz 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 326 KB 👁 2 views

## Abstract The chronic severe anemia of patients with β‐thalassemia major stimulates extensive erythropoiesis, which results in circulating nucleated normoblasts. We devised a dual staining flow cytometric procedure in order to analyse the cell cycle and ploidy of these normoblasts. Peripheral blo