To measure the concentrations of cytosolic ionized calcium in platelets we used a calcium-sensitive fluorophor (Fura-2) with two different spectrofluorometers (Perkin-Elmer LS-5 and Fluorolog 222). Values obtained by these two instruments for the basal cytosolic ionized calcium concentration of rest
Flow cytometric measurements of cytoplasmic calcium changes in human platelets
β Scribed by Theresa A. Davies; Daniel Drotts; Gary J. Weil; Elizabeth R. Simons
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 506 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0196-4763
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## Background: Platelet activation plays a major role in the physiology and pathology of hemostasis. flow cytometry is a promising approach for the structural and functional analysis of platelets. however, the choice of adequate biological parameters and most technical issues are still under discus
We measured the intracellular levels of free cytoplasmic calcium in Werent phases of the cell cycle in viable rat fibroblasts, using two parameter flow cytometric analysis with Hoechst 33342 as the DNA specific dye and Fluo-3 as the calcium sensitive dye. We studied changes in calcium levels during