Flow cytometric identification of cancer cells in effusions with ca1 monoclonal antibody

✍ Scribed by Bogdan Czerniak; Peter R. Papenhausen; Fritz Herz; Leopold G. Koss

• Book ID: 101328675
• Publisher: John Wiley and Sons
• Year: 1985
• Tongue: English
• Weight: 443 KB
• Volume: 55
• Category: Article
• ISSN: 0008-543X
• DOI: 10.1002/1097-0142(19850615)55:12<2783::aid-cncr2820551211>3.0.co;2-v

✦ Synopsis

The fluorescence of cells from 42 pleural and peritoneal effusions stained with Cal monoclonal antibody (CalMA) was studied by flow cytometry. In 14 of 17 malignant effusions a significantly higher intensity of fluorescence was observed in samples exposed to CalMA when compared with controls. There was no increase of fluorescence intensity in 25 benign effusions. The method failed in three malignant effusions: one due to endometrial carcinoma and two to malignant lymphoma. The sensitivity of the method was tested in experimental samples with a known percentage of malignant cells. The positive fluorescence with CalMA was detected in samples containing 0.1% of carcinoma cells. Flow cytometry with CalMA can be a relatively simple method of identification of malignant cells in effusions.