Flow cytometric evaluation of Thomsen-Friedenreich antigen on transitional cell cancer using monoclonal antibody

✍ Scribed by Oda, H. ;Oda, T. ;Ohoka, H. ;Yokoyama, M. ;Takeuchi, M.

• Publisher: Springer
• Year: 1990
• Tongue: English
• Weight: 526 KB
• Volume: 18
• Category: Article
• ISSN: 0300-5623
• DOI: 10.1007/bf00302469

✦ Synopsis

In 31 transitional cell cancer (TCC) tissues and 5 normal bladder mucosae (NBM), we compared the results of flow cytometry (FCM) and immunohistochemical examination in evaluating the expression of Thomsen-Friedenreich antigen (T-Ag) using a monoclonal antibody. On immunohistochemical examination, 14 (45%) cancer tissues showed T-Ag, while 7 (23%) cancer tissues and all NBM showed only cryptic T-Ag, which was detected only after neuraminidase treatment. Ten (32%) high grade cancer tissues showed neither T-Ag nor cryptic T-Ag. ON FCM the T-Ag positive cells (TPC) and the T-Ag positive cells after neuraminidase treatment (nTPC) were counted in fresh cell suspensions. FCM was more sensitive than immunohistochemical study in detecting T-Ag. Additionally, FCM revealed that some tumors had both T-Ag and cryptic T-Ag at the same time. The ratio of nTPC to TPC was well correlated with the stage or grade of the tumor and may be a more reliable marker of TCC than the expression of T-Ag assessed by immunohistochemical techniques.