We measured the intracellular levels of free cytoplasmic calcium in Werent phases of the cell cycle in viable rat fibroblasts, using two parameter flow cytometric analysis with Hoechst 33342 as the DNA specific dye and Fluo-3 as the calcium sensitive dye. We studied changes in calcium levels during
β¦ LIBER β¦
Flow cytometric identification and purification of cells by ligand-induced changes in intracellular calcium
β Scribed by Ira Schieren; Amy MacDermott
- Book ID
- 104171159
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 959 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0165-0270
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Flow cytometry (FCM) was used with different viability dyes to assess changes in cell structure and function induced by acetic acid (AA) in populations of Zygosaccharomyces bailii (AA resistant) and Saccharomyces cerevisiae (AA sensitive). Kinetic changes in esterase activity, intracellular dye proc