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Flow cytometric detection of intracellular expression of cytokines after activation of peripheral blood mononuclear cells (PMBC) in vitro

✍ Scribed by Jean-Marc, Ovigne; Annie, Bord; Pierre, Casellas; Géraldine, Petitpretre; Pierre, Carayon

Book ID
122246823
Publisher
Portland Press
Year
1992
Tongue
English
Weight
119 KB
Volume
76
Category
Article
ISSN
0248-4900

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Human peripheral blood mononuclear cells were incubated at 37 °C with bilirubin in bovine albumin solution. Histological analysis of bilirubin-treated cells demonstrated a prominent brown pigment deposited in the cytoplasm. Homogenates of these cells in chloroform-methanol solution showed an identic