𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Evaluation of the Suitability of Monoclonal Antibodies for Flow Cytometric Intracellular Cytokine Detection in Porcine Peripheral Blood Lymphocytes

✍ Scribed by Sipos, W. ;Willheim, M. ;Hofbauer, G. ;Pietschmann, P.


Book ID
115159305
Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
115 KB
Volume
52
Category
Article
ISSN
0931-184X

No coin nor oath required. For personal study only.


πŸ“œ SIMILAR VOLUMES


Permeabilization and fixation conditions
✍ Alan H. Lazarus; Janet Ellis; Victor Blanchette; John Freedman; Xiofang Sheng-Ta πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 173 KB πŸ‘ 2 views

Expression of the T-cell receptor (TCR) zeta chain in normal individuals was studied by two-color flow cytometric analysis using digitonin-permeabilized human peripheral blood lymphocytes. Optimal detection of the TCR zeta chain involved fixation of cells in 0.25% paraformaldehyde for 2 min and perm