Flow cytometric analysis of the uptake of hoechst 33342 dye by human lymphocytes
✍ Scribed by Gábor Szabó Jr.; Attila Kiss; Sándor Damjanovich
- Publisher
- John Wiley and Sons
- Year
- 1981
- Tongue
- English
- Weight
- 374 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0196-4763
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📜 SIMILAR VOLUMES
## Abstract A method of preparation and flow cytometric analysis of chromosomes from human peripheral lymphocytes is described. The procedure allows a resolution coefficient of variation better than 3% using propidium iodide staining and a commercially available flow cytometer.
Flow cytometric technique was used to study the effects of the fungicide Thiophanate-methyl on cell proliferation, micronucleus induction, and apoptosis in human peripheral blood lymphocytes treated in vitro. In particular, a combined approach of flow cytometry and fluorescence in situ hybridization