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Flavin binding by bacterial luciferase: Affinity chromatography of bacterial luciferase

โœ Scribed by C.A. Waters; J.R. Murphy; J.W. Hastings


Book ID
118309576
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
361 KB
Volume
57
Category
Article
ISSN
0006-291X

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The NADH-dependent and NADPH-dependent oxidoreductase activities associated with bacterial luciferase in Vibrio (Beneckea) harveyi can simultaneously be removed from purified luciferase through a Blue Sepharose CL-6B column. The result achieved with this one-step affinity chromatography is similar t