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Filter paper disk techniques for assay of nucleotidase

✍ Scribed by Wernere G. Müller; Heinz C. Schröder; Josef Arendes; Rudolf K. Zahn; Klaus Dose

Book ID
104768632
Publisher
Springer
Year
1977
Tongue
English
Weight
380 KB
Volume
3
Category
Article
ISSN
0301-4851

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✦ Synopsis

A DE filter disk technique for assaying the activity of nucleotidase is described. This method is based on the observation that nucleotides bind to the filters at 5 mM Tris-HCl (pH 7.8) while nucleosides do not. As parameter for the nucleotidase activity the decrease of bound nucleotides is determined. In parallel experiments the amount of the product (nucleoside) formed can be measured by DEAE Sephadex column chromatography. The filter disk technique can be applied for the determination of vmax and Km of a nucleotidase by using different ribonucleosidase monophosphate substrates.

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