Film formation and surface properties of enzymatically crosslinked casein films

✍ Scribed by Helka Juvonen; Maria Smolander; Harry Boer; Jaakko Pere; Johanna Buchert; Jouko Peltonen

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 809 KB
• Volume: 119
• Category: Article
• ISSN: 0021-8995
• DOI: 10.1002/app.32943

✦ Synopsis

Abstract

The use of renewable materials as barrier material is currently intensively investigated. Biopolymers such as polysaccharides, lipids, and proteins have been studied as barrier materials. Protein‐based films often possess good gas barrier properties, but because of their hydrophilic nature the gas barrier properties are sensitive to humidity. The improvement of the properties of sodium caseinate barrier films in potential packaging applications was studied by investigating the effects of enzymatic treatment and plasticizer on the film properties. Oxidoreductases Trametes hirsuta laccase (ThL) and Trichoderma reesei tyrosinase (TrTyr) were compared with transglutaminase for crosslinking of the sodium caseinate molecules in the films and coatings. All of the studied enzymes were able to crosslink sodium caseinate. Film solubility tests, protein electrophoresis, contact angle measurements, and atomic force microscopy studies showed that TrTyr treatment results in sodium caseinate films and coatings with better overall properties compared to treatment with ThL. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011