When using microvascular polytetrafluoroethylene (PTFE) vascular grafts, the best results in terms of patency rate and neoendothelialization are obtained with prostheses with thin walls and long fibril length (i.e., 90 microm). A complete internal neoendothelial lining is usually achieved at 12 weeks after implantation. Clinically, this period can be too long. In this study, 1-mm internal diameter PTFE prostheses with optimal physical characteristics were pretreated with basic fibroblast growth factor in fibrin glue, a potent endothelial cell mitogen, and chemoattractant. Rate, speed, extent, quality, and origin of neoendothelium were compared with two control groups, using Evans Blue dye, immunohistochemical localization of factor VIII von Willebrand factor protein, and scanning electron microscopy. Prostheses (8 mm long) were implanted in the infrarenal rat aorta and harvested after 3 weeks. In treated grafts, the amount of endothelial regeneration was greater than in untreated grafts (75% of the internal surface compared with 30%). However, patency rate in the experimental group was lower than in the control groups. This study provides new data on neoendothelial regeneration in small-diameter PTFE grafts.