Fibre-optic biosensor for hypoxanthine and xanthine based on a chemiluminescence reaction

✍ Scribed by J. Hlavay; S.D. Haemmerli; G.G. Guilbault

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 592 KB
• Volume: 9
• Category: Article
• ISSN: 0956-5663
• DOI: 10.1016/0956-5663(94)80121-5

✦ Synopsis

Fibre-optic biosensors were constructed for determination of hypoxanthine and xanthine. Xanthine oxidase and peroxidase were immobilized on different preactivated membranes which were subsequently mounted onto the tip of a fibre-optic bundle. The H202 generated by the reaction of hypoxanthine and xanthine oxidase was measured by chemiluminescence (CL) detection using luminol and peroxidase. A linear calibration curve of the sensors in the range of 1-316 PM hypoxanthine and 3.1-316 PM xanthine, respectively, with a detection limit of 0.55 PM hypoxanthine was obtained. Recovery of hypoxanthine ranged between 91 and 102%.