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Determination of sulphite by use of a fiber-optic biosensor based on a chemiluminescent reaction

✍ Scribed by József Hlavay; George G. Guilbault

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
568 KB
Volume
299
Category
Article
ISSN
0003-2670

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✦ Synopsis

A fiber-optic biosensor based on the immobilization of sulphite oxidase and peroxidase on different membranes, and subsequent detection of luminol chemiluminescence has been developed. The response was linear in the concentration ranges of 1 to 100 /_LM and 3.2 to 320 PM, with a detection limit (signal-to-noise ratio = 3) of 0.5 PM sulphite. A Nucleopore polycarbonate membrane proved to be superior to the chemically preactivated ones. The effect of pH, buffer, and immobilization conditions, as well as the elimination of ascorbic acid interference are also discussed. The feasibility of the biosensor developed was demonstrated by assaying the sulphite content of different food samples.

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