Feeder-free culture of human embryonic stem cells in conditioned medium for efficient genetic modification
β Scribed by Braam, Stefan R; Denning, Chris; Matsa, Elena; Young, Lorraine E; Passier, Robert; Mummery, Christine L
- Book ID
- 109946506
- Publisher
- Nature Publishing Group
- Year
- 2008
- Tongue
- English
- Weight
- 346 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1750-2799
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## Abstract The proven pluripotency of ES cells is expected to allow their therapeutic use for regenerative medicine. We present here a novel suspension culture method that facilitates the proliferation of pluripotent ES cells without feeder cells. The culture medium contains polyvinyl alcohol (PVA
## Abstract Realizing the potential clinical and industrial applications of human embryonic stem cells (hESCs) is limited by the need for costly, labile, or undefined growth substrates. Here we demonstrate that trypsin passaging of the hESC lines, HUES7 and NOTT1, on oxygen plasma etched tissue cul