Automated, scalable culture of human embryonic stem cells in feeder-free conditions
β Scribed by Rob J. Thomas; David Anderson; Amit Chandra; Nigel M. Smith; Lorraine E. Young; David Williams; Chris Denning
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 520 KB
- Volume
- 102
- Category
- Article
- ISSN
- 0006-3592
No coin nor oath required. For personal study only.
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## Abstract This study attempted to investigate whether different levels of mitotic activity exist within different physical regions of a human embryonic stem (hES) cell colony. Incorporation of 5βbromoβ2βdeoxyuridine (BrdU) within newlyβsynthesized DNA, followed by immunocytochemical staining was
## Abstract Realizing the potential clinical and industrial applications of human embryonic stem cells (hESCs) is limited by the need for costly, labile, or undefined growth substrates. Here we demonstrate that trypsin passaging of the hESC lines, HUES7 and NOTT1, on oxygen plasma etched tissue cul