𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Feasibility of ultra high performance supercritical neat carbon dioxide chromatography at conventional pressures

✍ Scribed by Cédric Sarazin; Didier Thiébaut; Patrick Sassiat; Jérôme Vial

Book ID
102446598
Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
175 KB
Volume
34
Category
Article
ISSN
1615-9306

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The implementation of columns packed with sub‐2 μm particles in supercritical fluid chromatography (SFC) is described using neat carbon dioxide as the mobile phase. A conventional supercritical fluid chromatograph was slightly modified to reduce extra column band broadening. Performances of a column packed with 1.8 μm C18‐bonded silica particles in SFC using neat carbon dioxide as the mobile phase were compared with results obtained in ultra high performance liquid chromatography (UHPLC) using a dedicated chromatograph. As expected and usual in SFC, higher linear velocities than in UHPLC must be applied in order to reach optimal efficiency owing to higher diffusion coefficient of solutes in the mobile phase; similar numbers of theoretical plates were obtained with both techniques. Very fast separations of hydrocarbons are presented using two different alkyl‐bonded silica columns.

📜 SIMILAR VOLUMES

Supercritical fluid carbon dioxide extra
Supercritical fluid carbon dioxide extraction of annatto seeds and quantification of trans-bixin by high pressure liquid chromatography
✍ Simon G. Anderson; Muraleedharan G. Nair; Amitabh Chandra; Errol Morrison 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 89 KB 👁 2 views

Bixin, a pigment used for food colouring is known to have a hyperglycemic effect. It was extracted using supercritical carbon dioxide, from the seeds of annatto (Bixa orellana L.). The major pigment in annotto, transbixin, was quantified by high pressure liquid chromatography. A combination of stati